Development of SSR primers using ISSR–PCR in Diospyros kaki Thunb.
Article first published online: 20 JUL 2006
Molecular Ecology Notes
Volume 6, Issue 3, pages 621–622, September 2006
How to Cite
GUO, D. L. and LUO, Z. R. (2006), Development of SSR primers using ISSR–PCR in Diospyros kaki Thunb. Molecular Ecology Notes, 6: 621–622. doi: 10.1111/j.1471-8286.2006.01341.x
- Issue published online: 14 AUG 2006
- Article first published online: 20 JUL 2006
- Received 27 September 2005; revision received 22 November 2005; accepted 2 February 2006
- Diospyros kaki Thunb.;
- SSR primer
Nine polymorphic single sequence repeat (SSR) primers were developed in Japanese persimmon using inter-SSR (ISSR) suppression polymerase chain reaction (PCR). These primers were tested on 30 individuals from Japan and China. The number of alleles per locus ranged from five to 20. Expected (HE) and observed (HO) heterozygosities at each locus ranged from 0.42 to 0.77 and 0.27 to 0.59, respectively. The SSR primers developed herein could be applied to cultivar identification, estimation of genetic diversity and divergence in Diospyros spp.