Present adress: Departament de Genètica Vegetal, Institut de Recerca i Tecnologia Agroalimentaria, Centre de Cabrils, Ctra de Cabrils s/n, 08348 Cabrils, Barcelona, Spain.
Development of SSR markers located in the G1 linkage group of apricot (Prunus armeniaca L.) using a bacterial artificial chromosome library
Article first published online: 24 MAY 2006
Molecular Ecology Notes
Volume 6, Issue 3, pages 789–791, September 2006
How to Cite
VILANOVA, S., SORIANO, J. M., LALLI, D. A., ROMERO, C., ABBOTT, A. G., LLÁCER, G. and BADENES, M. L. (2006), Development of SSR markers located in the G1 linkage group of apricot (Prunus armeniaca L.) using a bacterial artificial chromosome library. Molecular Ecology Notes, 6: 789–791. doi: 10.1111/j.1471-8286.2006.01346.x
S. Vilanova and J. M. Soriano contributed equal to this work.
- Issue published online: 14 AUG 2006
- Article first published online: 24 MAY 2006
- Received 5 January 2006; revision accepted 30 January 2006
- BAC library;
- Prunus armeniaca;
Sixteen simple sequence repeats (SSRs) of apricot (Prunus armeniaca L.) were isolated from a bacterial artificial chromosome (BAC) library. Twelve restriction fragment length polymorphism (RFLP) probes mapped on LG1 of the Prunus general map were hybridized to the BAC library in order to select clones belonging to G1 linkage group of apricot. Selected BACs were digested, subcloned and hybridized with probes containing repeat motifs (GA)10 and (TA)10. Sequencing of the positive subclones revealed 18 unique SSR sequences of which 16 allowed the design of primers flanking the SSR. From the 16 primer pairs, 10 amplified polymorphic markers with an average of observed and expected heterozygosities of 0.44 and 0.68, respectively. The procedure described here proves to be a useful technique for obtaining markers in target areas of a genome.