Thirteen polymorphic microsatellite markers were developed in rose (Rosa hybrida L.) from an enriched genomic library. Ten out of 13 microsatellites showed amplification and produced four to 12 polymorphic alleles per locus, with an average of 8.4 in Rosa multiflora. The average values of observed and expected heterozygosities among the 10 loci were 0.62 and 0.82, respectively. Thirteen microsatellites produced three to 22 genotypes with an average value of 11.4 in modern garden roses. The average value of the power of discrimination among the microsatellites was 0.79, ranging from 0.29 to 0.95.