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Keywords:

  • DNA;
  • groupers;
  • microsatellite loci;
  • polymorphic;
  • Serranidae

Abstract

We characterized nine microsatellite loci and identified an additional 60 genomic regions containing microsatellites in the red hind grouper, Epinephelus guttatus. The nine loci were highly polymorphic, and primers designed from red hind genomic DNA produced a strong amplification product in a test panel of 16 other groupers in the genera Epinephelus and Mycteroperca collected from across the world. Most of the amplified regions were homologous to the red hind locus and a well-defined microsatellite repeat was generally evident. The nine loci, together with the 60 uncharacterized microsatellite-containing regions, provide a powerful tool for ecological and evolutionary studies in groupers.