Characterization of microsatellite loci in Brachyderes rugatus, the Canary Islands pine weevil (Coleoptera: Curculionidae)

Authors

  • SHAUN A. FORGIE,

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    • Present addresses: The Horticulture and Food Research Institute of New Zealand Ltd., 120 Mt. Albert Road, Private Bag 92 169, Auckland, New Zealand.

  • SARA L. GOODACRE,

    1. Centre for Ecology, Evolution and Conservation (CEEC), School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, Norfolk, UK
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  • MARTIN I. TAYLOR,

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    • Present addresses: School of Biological Sciences, University of Wales, Bangor, Gwynedd LL57 2UW, UK.

  • BRENT C. EMERSON

    1. Centre for Ecology, Evolution and Conservation (CEEC), School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, Norfolk, UK
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Sara Goodacre, Fax: +44-1608-592250; E-mail: s.goodacre@uea.ac.uk

Abstract

The Canary Island pine weevil Brachyderes rugatus (Wollaston) consists of four allopatric subspecies that are thought to have arisen from several historic colonization events within the archipelago. We have isolated and optimized seven microsatellite loci from Brachyderes rugatus calvus from Gran Canaria. Six of these loci are polymorphic within B. rugatus (11–22 alleles per locus; heterozygosity between 0.43 and 0.84). There is no evidence for heterozygote deficit within populations or for linkage disequilibrium between pairs of loci. These molecular markers are likely to prove useful tools for quantifying the genetic variability of bottlenecked island populations.

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