Isolation and characterization of microsatellites in giant freshwater prawn Macrobrachium rosenbergii

Authors


Supawadee Poompuang, Fax: 66-2-5613984; E-mail: supawadee.p@ku.ac.th

Abstract

Microsatellite loci were characterized in a freshwater prawn from enriched genomic library using six biotinylated probes: (AG)10, (TG)10, (CAA)10, (CAG)10, (GAT)10 and (TAC)10. Primers for DNA amplification were designed and synthesized for 20 loci. Ten loci were polymorphic with the number of alleles ranging from five to 17 alleles per locus and the observed heterozygosity ranging from 0.27 to 0.83 per locus. Developed microsatellite primers should prove useful for selective breeding programs and population genetic studies of freshwater prawn.

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