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Keywords:

  • DNA pedigree;
  • enrichment;
  • parentage;
  • pearl culture;
  • population genetics

Abstract

Six di-, tri- and tetranucleotide microsatellite loci were developed for the silver- or gold-lipped pearl oyster Pinctada maxima using a linker-ligated, magnetic bead enrichment protocol. Based on a minimum of 134 Indonesian pearl oyster samples, number of alleles and observed heterozygosity at each locus ranged from six to 17 alleles and from 0.172 to 0.813 (mean = 0.448), respectively. Mean polymorphic information content for the six loci was 0.562. These loci should be very useful in DNA parentage analyses and population differentiation of P. maxima in Australia and Indonesia.