Present address: INRA, UGAFL, Domaine St Maurice, BP 94, 84143 Montfavet cedex, France.
Identification and characterization of nuclear microsatellites in Mediterranean cedars (Cedrus sp.)
Article first published online: 27 APR 2006
Molecular Ecology Notes
Volume 6, Issue 3, pages 840–842, September 2006
How to Cite
CHAIB, J., DANAN, S., JOUAUD, B., HAGEN, L. S., LEFEVRE, F. and FADY, B. (2006), Identification and characterization of nuclear microsatellites in Mediterranean cedars (Cedrus sp.). Molecular Ecology Notes, 6: 840–842. doi: 10.1111/j.1471-8286.2006.01364.x
- Issue published online: 14 AUG 2006
- Article first published online: 27 APR 2006
- Received 12 January 2006; revision accepted 17 February 2006
- DNA library;
We developed primers for the amplification of six polymorphic nuclear microsatellites in Mediterranean Cedrus taxa. Microsatellites originated from two Cedrus atlantica genomic libraries enriched for TC (four markers) and TG (two markers) motifs. No distortion from Mendelian segregation was observed. There were up to eight alleles per locus and mean expected heterozygosity was 0.75. Four microsatellites also amplified in the Himalayan cedar. These markers should be helpful for species identification, diversity studies, parentage analysis and genome mapping and to monitor biodiversity in endangered Mediterranean Cedrus forests.