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Isolation of polymorphic tetranucleotide microsatellite markers for the green-eyed tree frog (Litoria genimaculata)

Authors

  • MELISSA M. GRAY,

    1. Department of Ecology and Evolutionary Biology, University of California, Los Angeles, 621 Charles Young Drive South, Los Angeles, California 90095-1606, USA,
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  • CONRAD J. HOSKIN,

    1. School of Integrative Biology, University of Queensland, St Lucia, Queensland 4072, Australia
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  • CAROLYNE BARDELEBEN

    1. Department of Ecology and Evolutionary Biology, University of California, Los Angeles, 621 Charles Young Drive South, Los Angeles, California 90095-1606, USA,
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Carolyne Bardeleben, Fax: 310-206-3987; E-mail: carolyne@ucla.edu

Abstract

We have isolated 16 polymorphic microsatellite markers for the green-eyed tree frog, Litoria genimaculata, from genomic libraries enriched for (AAGG)n and (AAAG)n repetitive elements. The number of alleles ranges from four to 14 per locus with the observed heterozygosity ranging from 0.36 to 1.00. These markers will be useful for analysis of questions concerning population genetic structure and speciation.

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