SEARCH

SEARCH BY CITATION

Keywords:

  • cross-species amplification;
  • microsatellite;
  • RAPD;
  • Scophthalmus maximus;
  • turbot

Abstract

Five polymorphic microsatellite loci have been isolated and characterized from random amplified polymorphic DNA product in turbot, Scophthalmus maximus. Twelve microsatellites were selected for designing microsatellite primers, of which five gave working primer pairs. They had between four and nine alleles. Observed and expected heterozygosities varied from 0.76 to 0.90 and from 0.63 to 0.83, respectively. Five additional fish species assessed for cross-species amplification revealed between one and three positive amplifications and between zero and two polymorphic loci per species.