Development of microsatellite markers for the red-listed wood-decay fungus Phlebia centrifuga

Authors

  • INA FRANZÉN,

    1. Department of Forest Mycology and Pathology, Swedish University of Agricultural Sciences, Uppsala, Sweden;
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  • BERNARD SLIPPERS,

    1. Department of Forest Mycology and Pathology, Swedish University of Agricultural Sciences, Uppsala, Sweden;
    2. The Forestry and Agricultural Biotechnology Institute, University of Pretoria, Pretoria, South Africa
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  • RIMVYDAS VASILIAUSKAS,

    1. Department of Forest Mycology and Pathology, Swedish University of Agricultural Sciences, Uppsala, Sweden;
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  • JAN STENLID

    1. Department of Forest Mycology and Pathology, Swedish University of Agricultural Sciences, Uppsala, Sweden;
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Ina Franzén, Fax: 46 18 673599; E-mail: ina.franzen@mykopat.slu.se

Abstract

Seven polymorphic microsatellite markers were developed for the wood-decay basidiomycete Phlebia centrifuga. The primers were identified using two techniques, based on intersimple sequence repeats (ISSR) and amplified fragment length polymorphism (AFLP), respectively. The markers were screened on 27 isolates from Europe and North America. Two markers varied only on a worldwide scale, but not within Europe. The other five showed variation on both scales. These markers will now be used to characterize populations of P. centrifuga, which is red-listed as near-threatened in its natural habitat due to human disturbance.

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