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Limited cross-species microsatellite amplification and the isolation and characterization of new microsatellite markers for the greater stick-nest rat (Leporillus conditor)

Authors


S.D. Barclay, Fax: +61 2 9385 1225; E-mail: shaun.barclay@unsw.edu.au

Abstract

The greater stick-nest rat (Leporillus conditor) is a conilurid rodent whose recent history provides an opportunity to examine genetic changes in reintroduced populations. We trialled 63 known microsatellite primers from Rattus rattus and Mus musculus in L. conditor. Three primer pairs produced polymorphic loci (number of alleles = 2–3, mean, HE = 0.42). Subsequently, we isolated and characterized 12 novel polymorphic microsatellites (mean number of alleles = 5–16, mean HE = 0.76) from L. conditor from genomic libraries for use in population genetic studies.

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