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Keywords:

  • Diospyros kaki Thunb.;
  • ISSR;
  • SSR primer

Abstract

Nine polymorphic simple sequence repeat (SSR) primers were developed in Japanese persimmon using inter-SSR (ISSR) suppression polymerase chain reaction (PCR). These primers were tested on 30 individuals from Japan and China. The number of alleles per locus ranged from five to 20. Expected and observed heterozygosities at each locus ranged from 0.42 to 0.77 and from 0.27 to 0.59, respectively. The SSR primers developed herein could be applied to cultivar identification, estimation of genetic diversity and divergence in Diospyros spp.