Get access

Isolation of polymorphic tetranucleotide microsatellite markers for the ornate nursery frog Cophixalus ornatus

Authors

  • CAROLYNE BARDELEBEN,

    1. Department of Ecology and Evolutionary Biology, University of California, Los Angeles, 621 Charles E. Young Drive South, Los Angeles, California 90095-1606, USA,
    Search for more papers by this author
  • MELISSA M. GRAY,

    1. Department of Ecology and Evolutionary Biology, University of California, Los Angeles, 621 Charles E. Young Drive South, Los Angeles, California 90095-1606, USA,
    Search for more papers by this author
  • CONRAD J. HOSKIN

    1. School of Integrative Biology, University of Queensland, St. Lucia 4072, Brisbane, Qucensland, Australia
    Search for more papers by this author

Carolyne Bardeleben, Fax: 310-206-3987; E-mail: carolyne@ucla.edu

Abstract

We have isolated 18 polymorphic microsatellite loci for Cophixalus ornatus from genomic libraries enriched for (AAAG)n, (AACC)n and (AAGG)n repetitive elements. The number of alleles ranges from five to 22 per locus with the observed heterozygosity ranging from 0.10 to 0.92. These markers will be useful for the analysis of population structure in C. ornatus and testing alternative models of speciation.

Ancillary