Characterization of microsatellite markers for rough fescue species (Festuca spp.)

Authors

  • YONG-BI FU,

    1. Plant Gene Resources of Canada, Saskatoon Research Centre, Agriculture and Agri-Food Canada, 107 Science Place, Saskatoon, Saskatchewan, Canada S7N 0X2
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  • JIE QIU,

    1. Plant Gene Resources of Canada, Saskatoon Research Centre, Agriculture and Agri-Food Canada, 107 Science Place, Saskatoon, Saskatchewan, Canada S7N 0X2
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  • GREGORY W. PETERSON,

    1. Plant Gene Resources of Canada, Saskatoon Research Centre, Agriculture and Agri-Food Canada, 107 Science Place, Saskatoon, Saskatchewan, Canada S7N 0X2
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  • WALTER D. WILLMS,

    1. Lethbridge Research Centre, Agriculture and Agri-Food Canada, PO Box 3000, Lethbridge, Alberta, Canada T1J 4B1
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  • JOHN F. WILMSHURST

    1. Western and Northern Service Centre, Parks Canada, 145 McDermot Avenue, Winnipeg, Manitoba, Canada R3B 0R9
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Yong-Bi Fu, Fax: +1306-956-7642; E-mail: fuy@agr.gc.ca

Abstract

One major challenge in genetic diversity analysis of minor grass species is the lack of informative molecular markers. A set of 210 simple sequence repeat (SSR) markers developed from wheat and barley were evaluated for their transferability to three rough fescue species [Festuca altaica Trinius, F. campestris (Rydb.) and F. hallii (Vassey) Piper]. Twelve SSR primer pairs displayed scorable polymorphism among and within the species. The number of alleles per primer pair ranged from three to 17 with an average of 8.3 for all the species and greatly varied for each species. About 82% of SSR variation resided within the species. Festuca hallii was genetically most distinct among the three species.

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