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Development and localization of microsatellite markers for the sibling species Chironomus riparius and Chironomus piger (Diptera: Chironomidae)

Authors

  • CARSTEN NOWAK,

    1. Department of Ecology and Evolution, J. W. Goethe-Universität, Siesmayerstr. 70, D-60054 Frankfurt am Main, Germany,
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  • THOMAS HANKELN,

    1. Institut für Molekulargenetik, Gentechnologische Sicherheitsforschung und Beratung, Johannes Gutenberg-Universität Mainz, Mainz, Germany,
    2. GENterprise GmbH, J.-J. Becherweg 32, D-55099 Mainz, Germany
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  • ERWIN R. SCHMIDT,

    1. Institut für Molekulargenetik, Gentechnologische Sicherheitsforschung und Beratung, Johannes Gutenberg-Universität Mainz, Mainz, Germany,
    2. GENterprise GmbH, J.-J. Becherweg 32, D-55099 Mainz, Germany
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  • KLAUS SCHWENK

    1. Department of Ecology and Evolution, J. W. Goethe-Universität, Siesmayerstr. 70, D-60054 Frankfurt am Main, Germany,
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Carsten Nowak, Fax: +4979824910; E-mail: c.nowak@zoology.uni-frankfurt.de

Abstract

Five variable microsatellite loci are reported for the nonbiting midge species Chironomus riparius and Chironomus piger. All loci show considerable intraspecific variation and species-specific alleles, which allow to discriminate among the two closely related species and their interspecific hybrids, and to estimate genetic diversity within and between populations. Additionally, the loci were localized on C. riparius polytene chromosomes to verify their single copy status and investigate possible chromosomal linkage. The described markers are used in different studies with regard to population and ecological genetics and evolutionary ecotoxicology of Chironomus.

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