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Isolation and characterization of microsatellite loci from the rust pathogen, Melampsora lini

Authors


L. G. Barrett, Fax: (+61) 2-6246-5000; E-mail: Luke.Barrett@csiro.au

Abstract

We developed and characterized primers for 11 variable microsatellite loci present in the genome of the flax rust, Melampsora lini. The microsatellite loci were identified by sequencing clones from a library of EcoRI DNA fragments enriched for four simple sequence repeat motifs (AAG, AAT, TC and TG). All 11 primer pairs successfully amplified DNA fragments from a sample of 102 M. lini isolates (98 isolated from Linum marginale and four from Linum usitatissimum), revealing a total of 32 alleles. Allelic diversity at the 11 loci ranged from 0.030 to 0.449.

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