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Isolation and characterization of eight microsatellite loci in Leporinus macrocephalus (Characiformes: Anostomidae) and cross-species amplification

Authors


Karina A. Morelli, Fax: 55 14 38116264; E-mail: kmorelli@ibb.unesp.br

Abstract

Leporinus macrocephalus is a species endemic to the Paraguay River basin and an important fishery resource, as well as a valuable species in aquaculture programs. A total of eight polymorphic microsatellite loci were isolated and characterized. A population survey was conducted involving 45 specimens whereby a large number of alleles (range 5–17 among loci), a highly observed (0.1667–0.6129) and an expected (0.6967–0.9448) heterozygosity was detected, indicating its usefulness in population genetics studies. Cross-species amplification was successful in eight Characiformes species.

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