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Keywords:

  • Charadriiformes;
  • Charadrius alexandrinus;
  • cross-species amplification;
  • microsatellite;
  • shorebirds

Abstract

We isolated 45 new Kentish plover (Charadrius alexandrinus) microsatellite loci. These were tested for polymorphism in 42 Kentish plovers breeding in the Çukurova Delta, Turkey. Thirty-six of the 45 loci were polymorphic with observed heterozygosity varying between 0.22 and 0.93. Genotypes of individuals of known sex indicated that two loci were sex-linked (Calex-26 is located on the Z chromosome and Calex-31 on the W chromosome). Additionally, we tested all loci for amplification in four other species of Charadridae (Kittlitz's plover, Madagascar plover, three-banded plover and white-fronted plover). On average 34 loci amplified per species (range 29–36).