Isolation of polymorphic microsatellite loci from the flea beetle Phyllotreta nemorum L. (Coleoptera: Chrysomelidae)


  • P. Verbaarschot and D. Calvo contributed equally to the paper.

P. Verbaarschot, Fax: +31 (0) 317 48 48 21; E-mail:


Ten microsatellite markers for the flea beetle Phyllotreta nemorum were developed using di- and trinucleotide repeat-enriched libraries. Each of these primer pairs were characterized on 96 individuals. Expected heterozygosities ranged between 0.11 and 0.84 and the number of alleles ranged between two and 14 per locus. These microsatellite markers are the first published for any Phyllotreta species.