Isolation of polymorphic tetranucleotide microsatellite markers for the silky short-tailed bat Carollia brevicauda

Authors

  • CAROLYNE BARDELEBEN,

    1. Department of Ecology and Evolutionary Biology, University of California, Los Angeles, 621 Charles E. Young Drive South, Los Angeles, California 90095-1606, USA,
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  • POLLY CAMPBELL,

    1. Department of Biology, Boston University, Boston, Massachusetts 02215, USA
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    • Present address: Department of Zoology, University of Florida, Gainesville, Florida 32611-8525, USA

  • MARCIA LARA,

    1. Department of Biology, Boston University, Boston, Massachusetts 02215, USA
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  • RACHAEL L. MOORE

    1. Department of Ecology and Evolutionary Biology, University of California, Los Angeles, 621 Charles E. Young Drive South, Los Angeles, California 90095-1606, USA,
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Carolyne Bardeleben, Fax: 310-206-3987; Email: carolyne@ucla.edu

Abstract

We isolated 10 polymorphic microsatellite markers for the silky short-tailed bat, Carollia brevicauda, from genomic libraries enriched for (AAGG)n repetitive elements. The number of alleles ranged from six to 25 per locus with the observed heterozygosity ranging from 0.29 to 0.95. These markers will be useful for analysis of questions concerning population genetic structure and models of speciation. Results of cross-species amplification in Carollia castanea and Carollia perspicillata are also reported.

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