Polymorphic microsatellite markers for studies of Aedes aegypti (Diptera: Culicidae), the vector of dengue and yellow fever

  1. M. A. SLOTMAN1,
  2. N. B. KELLY1,
  3. L. C. HARRINGTON2,
  4. S. KITTHAWEE3,
  5. J. W. JONES4,
  6. T. W. SCOTT5,
  7. A. CACCONE1,
  8. J. R. POWELL1

Article first published online: 21 SEP 2006

DOI: 10.1111/j.1471-8286.2006.01533.x

Issue

Molecular Ecology Notes

Molecular Ecology Notes

Volume 7, Issue 1, pages 168–171, January 2007

Additional Information

How to Cite

SLOTMAN, M. A., KELLY, N. B., HARRINGTON, L. C., KITTHAWEE, S., JONES, J. W., SCOTT, T. W., CACCONE, A. and POWELL, J. R. (2007), Polymorphic microsatellite markers for studies of Aedes aegypti (Diptera: Culicidae), the vector of dengue and yellow fever. Molecular Ecology Notes, 7: 168–171. doi: 10.1111/j.1471-8286.2006.01533.x

Author Information

  1. 1

    Department of Ecology and Evolutionary Biology, Yale University, New Haven, CT 06250-8106, USA,

  2. 2

    Department of Entomology, Cornell University, Ithaca, NY 14853, USA,

  3. 3

    Department of Biology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand,

  4. 4

    Department of Entomology, US Medical Component, Armed Forces Research Institute of Medical Sciences, Bangkok 10400, Thailand,

  5. 5

    Department of Entomology, University of California-Davis, Davis, CA 95616, USA

* Michel A. Slotman, Fax: 203 4325176; E-mail: michel.slotman@yale.edu

Publication History

  1. Issue published online: 9 NOV 2006
  2. Article first published online: 21 SEP 2006
  3. Received 9 May 2006; revision received 16 June 2006; accepted 25 July 2006

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Keywords:

  • Aedes aegypti;
  • dengue vector;
  • genetic markers;
  • microsatellites;
  • population genetics

Abstract

A significant challenge to population genetic studies of the dengue vector, Aedes aegypti, has been the lack of polymorphic microsatellite loci. In an effort to develop useful markers, we evaluated the genetic variation at 17 microsatellite loci identified in the A. aegypti genome. Nine loci with at least five alleles were identified in field-collected specimens from Thailand. An additional two loci carried five alleles if samples from an A. aegypti laboratory colony were included. Our results greatly increase the number of highly variable markers available for the study of the genetics and the population structure of this medically important species.

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