Present address: Malaria Research Unit, Department of Parasitology, Faculty of Medicine, University of Colombo, Kynsey Road, Colombo 8, Sri Lanka.
Fourteen polymorphic microsatellite DNA markers for the human malaria parasite Plasmodium vivax
Version of Record online: 20 SEP 2006
Molecular Ecology Notes
Volume 7, Issue 1, pages 172–175, January 2007
How to Cite
KARUNAWEERA, N. D., FERREIRA, M. U., HARTL, D. L. and WIRTH, D. F. (2007), Fourteen polymorphic microsatellite DNA markers for the human malaria parasite Plasmodium vivax. Molecular Ecology Notes, 7: 172–175. doi: 10.1111/j.1471-8286.2006.01534.x
- Issue online: 9 NOV 2006
- Version of Record online: 20 SEP 2006
- Received 9 May 2006; revision received 16 June 2006; accepted 25 July 2006
- genetic diversity;
- Plasmodium vivax ;
- Sri Lanka
We have optimized a set of 14 polymorphic microsatellite markers for the human malaria parasite Plasmodium vivax, all of them consisting of either tri- or tetranucleotide repeats. These markers, whose polymerase chain reaction amplification conditions are identical, were used to screen 25 parasite isolates from malaria-endemic areas in Sri Lanka. The total number of alleles per locus ranged between 6 and 13 (average, 7.8), and expected heterozygosity ranged from 0.627 to 0.913 (average, 0.790). These markers are now being used to characterize the population structure of P. vivax in other endemic areas.