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Genetically diversified populations in the loach Misgurnus anguillicaudatus inferred from newly developed microsatellite markers


Lenin Arias Rodriguez, Fax: +81-138-40-5537; E-mail:


The loach Misgurnus anguillicaudatus is a freshwater fish which include natural polyploids and clones besides most bisexually reproducing diploids. Recent genetic studies using allozymes differentiated the populations in northern region of Hokkaido from other populations in Japan. Here, we developed 23 new sets of polymerase chain reaction primers to detect polymorphic microsatellites loci and tested them in 20 individuals from Memanbetsu town (northern Hokkaido) and Kita village (southern Hokkaido). These markers can be potentially diagnostic for identification of two populations.