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Characterization of microsatellite DNA markers for a mahseer species, Tor tambroides (Cyprinidae) and cross-amplification in four congeners

Authors

  • THUY NGUYEN,

    1. Network of Aquaculture Centres in Asia-Pacific, PO Box 1040, Kasetsart Post Office, Bangkok 10903, Thailand,
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  • MATTHEW BARANSKI,

    1. AKVAFORSK (Institute for Aquaculture Research AS), N-1432 Ås, Norway,
    2. Animal Genetics and Genomics, Department of Primary Industries, 475 Mickleham Road, Attwood, Victoria 3049, Australia
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  • MEAGHAN ROURKE,

    1. Animal Genetics and Genomics, Department of Primary Industries, 475 Mickleham Road, Attwood, Victoria 3049, Australia
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  • HELEN McPARTLAN

    1. Animal Genetics and Genomics, Department of Primary Industries, 475 Mickleham Road, Attwood, Victoria 3049, Australia
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Thuy Nguyen, Fax: +66 25611727; E-mail: thuy.nguyen@enaca.org

Abstract

This study reports the isolation and characterization of microsatellite DNA markers in a mahseer species, Tor tambroides (Pisces, Cyprinidae). Of a total of 14 loci evaluated, 10 were polymorphic in T. tambroides samples, with an average of 2.86 alleles per locus. Deviations from Hardy–Weinberg equilibrium were observed at one locus and there was no indication of linkage disequilibrium among loci. A high level of cross-amplification among four congeners was achieved, with 12 loci successfully amplifying and 11 loci showing polymorphism in at least one other species. These markers will be a useful resource for population genetic studies and broodstock management of closely related mahseer species.

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