We report here on the development of six polymorphic microsatellite loci for Labiotermes labralis. This soil-feeding species is restricted to the Neotropical rainforest and then could represent a major candidate for being a bio-indicator of anthropic disturbance resulting in the forest fragmentation. The microsatellite loci were isolated and characterized using a microsatellite-enriched genomic library. The primers were tested on a French Guyanese population of L. labralis, represented by the sampling of one soldier in 22 nests. The primers were also tested against nine species of the same Nasutitermitinae subfamily and were successfully amplified at five loci in Armitermes minutus.