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Isolation and characterization of microsatellite markers in the caddisfly Drusus discolor (Trichoptera: Limnephilidae)

Authors

  • STEFFEN U. PAULS,

    1. The Field Museum, Pritzker Laboratory for Molecular Systematics and Evolution, 1400 South Lake Shore Drive, Chicago, IL 60605, USA,
    2. Research Institute Senckenberg, Department of Limnology and Conservation Research, Clamecystrasse 12, 63571 Gelnhausen, Germany
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  • KEVIN A. FELDHEIM,

    1. The Field Museum, Pritzker Laboratory for Molecular Systematics and Evolution, 1400 South Lake Shore Drive, Chicago, IL 60605, USA,
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  • PETER HAASE

    1. Research Institute Senckenberg, Department of Limnology and Conservation Research, Clamecystrasse 12, 63571 Gelnhausen, Germany
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Steffen U. Pauls, Fax: +49 (6051) 9169713; E-mail: steffen.pauls@senckenberg.de

Abstract

We describe the development of and amplification conditions for microsatellite primers isolated from the caddisfly Drusus discolor. Eight polymorphic microsatellite loci were developed and screened for variability using 37 individuals from two populations from central Europe. The primers yielded an average of 8.6 alleles per loci. No linkage disequilibrium between loci was detected, while three loci showed deviations from Hardy–Weinberg equilibrium in one of the two tested populations.

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