Isolation and characterization of microsatellite markers in pangolins (Mammalia, Pholidota, Manis spp.)

  1. SHU-JIN LUO1,2,
  2. QING-XIU CAI3,
  3. VICTOR A. DAVID1,
  4. LI ZHANG3,
  5. PAOLO MARTELLI4,
  6. NORMAN T-L. LIM5,
  7. NUNO FERRAND6,
  8. SHIH-CHIEN CHIN7,
  9. PHILIPPE GAUBERT8,
  10. MARIA JOÃO RAMOS9,
  11. STEPHEN J. O’BRIEN1,
  12. AGOSTINHO ANTUNES1,9,
  13. WARREN E. JOHNSON1

Article first published online: 29 NOV 2006

DOI: 10.1111/j.1471-8286.2006.01577.x

Issue

Molecular Ecology Notes

Molecular Ecology Notes

Volume 7, Issue 2, pages 269–272, March 2007

Additional Information

How to Cite

LUO, S.-J., CAI, Q.-X., DAVID, V. A., ZHANG, L., MARTELLI, P., LIM, N. T.-L., FERRAND, N., CHIN, S.-C., GAUBERT, P., RAMOS, M. J., O’BRIEN, S. J., ANTUNES, A. and JOHNSON, W. E. (2007), Isolation and characterization of microsatellite markers in pangolins (Mammalia, Pholidota, Manis spp.). Molecular Ecology Notes, 7: 269–272. doi: 10.1111/j.1471-8286.2006.01577.x

Author Information

  1. 1

    Laboratory of Genomic Diversity, National Cancer Institute, Frederick, MD 21702-1201, USA,

  2. 2

    Department of Fisheries, Wildlife and Conservation Biology, University of Minnesota, St. Paul, MN 55108, USA,

  3. 3

    Key Laboratory for Biodiversity Science and Ecological Engineering, Ministry of Education; Institute of Ecology, Beijing Normal University, Beijing 100875, China,

  4. 4

    Ocean Park, Aberdeen, Hong Kong,

  5. 5

    Systematics and Ecology Laboratory, Department of Biological Sciences, National University of Singapore, Singapore 117543,

  6. 6

    Centro de Investigação em Biodiversidade e Recursos Genéticos (CIBIO/UP), Campus Agrário de Vairão, 4485-661 Vairão, Portugal; and Departamento de Zoologia-Antropologia, Faculdade de Ciências, Universidade do Porto, Praça Gomes Teixeira, 4099-002 Porto, Portugal,

  7. 7

    Taipei Zoo, 30 Sec. 2 Hsin Kuang Road, Taipei, Taiwan 1168,

  8. 8

    Institut de Recherche pour le Développement, UR136 ‘Aires Protégées’, TechnoParc 5, rue du Carbone, 45072 Orléans, Cedex 2, France,

  9. 9

    REQUIMTE, Departamento de Química, Faculdade de Ciências, Universidade do Porto, Rua do Campo Alegre, 687, 4169-007 Porto, Portugal

* Agostinho Antunes. Fax: +351 22 6082959; E-mail: aantunes@ncifcrf.gov; and Warren E. Johnson. Fax: +301-846-6327; E-mail: johnsonw@mail.ncifcrf.gov

Publication History

  1. Issue published online: 20 DEC 2006
  2. Article first published online: 29 NOV 2006
  3. Received 13 August 2006; revision received 18 September 2006; accepted 18 September 2006

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Keywords:

  • Manis;
  • microsatellite;
  • pangolin;
  • Pholidota

Abstract

Thirty-four polymorphic dinucleotide microsatellite loci were developed in the Malayan pangolin Manis javanica. Of the 34 markers, 32 and 18 were also amplified, respectively, in the Chinese pangolin (Manis pentadactyla) and the African tree pangolin (Manis tricuspis). Analysis of 24 Malayan, 12 Chinese and 2 African tree pangolins showed high levels of variability (heterozygosity ranging from 0.321 to 0.708). These are the first available microsatellite markers in Pholidota and will be an invaluable tool for evolutionary and conservation genetic studies in pangolins.

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