PRIMER NOTE

Seventy microsatellite markers from Persea americana Miller (avocado) expressed sequence tags

  1. JAMES W. BORRONE1,
  2. RAYMOND J. SCHNELL1,
  3. HELEN A. VIOLI2,
  4. RANDY C. PLOETZ2

Article first published online: 22 DEC 2006

DOI: 10.1111/j.1471-8286.2006.01611.x

Issue

Molecular Ecology Notes

Molecular Ecology Notes

Volume 7, Issue 3, pages 439–444, May 2007

Additional Information

How to Cite

BORRONE, J. W., SCHNELL, R. J., VIOLI, H. A. and PLOETZ, R. C. (2007), Seventy microsatellite markers from Persea americana Miller (avocado) expressed sequence tags. Molecular Ecology Notes, 7: 439–444. doi: 10.1111/j.1471-8286.2006.01611.x

Author Information

  1. 1

    United States Department of Agriculture, Subtropical Horticulture Research Station, National Germplasm Repository, 13601 Old Cutler Road, Miami, FL 33158, USA,

  2. 2

    Department of Plant Pathology, University of Florida, Institute of Food and Agricultural Sciences, Tropical Research and Education Center, 18905 SW 280th Street, Homestead, FL 33031, USA

* Raymond J. Schnell, Fax: 305-969-6410; E-mail: rschnell@saa.ars.usda.gov

Publication History

  1. Issue published online: 22 DEC 2006
  2. Article first published online: 22 DEC 2006
  3. Received 23 August 2006; revision accepted 15 October 2006

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Keywords:

  • expressed sequence tag libraries;
  • Persea americana Miller;
  • simple sequence repeat;
  • unigene

Abstract

Expressed sequence tags for Persea americana Mill. were investigated to expand upon the number of informative microsatellite markers available for avocado. Seventy informative loci were discovered using 24 P. americana var. americana Mill. accessions. The number of alleles detected ranged from two to 17 and averaged 7.1 alleles per locus. These primers successfully amplified products in different varieties of P. americana, hybrids and a related species, Persea schiedeana. These primers will be useful for characterizing germplasm, determining genetic relationships of cultivated accessions, and for marker-assisted development of root rot-tolerant P. americana var. americana rootstock material.

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