DNA barcoding of Neotropical bats: species identification and discovery within Guyana
Article first published online: 12 JAN 2007
DOI: 10.1111/j.1471-8286.2006.01657.x
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How to Cite
CLARE, E. L., LIM, B. K., ENGSTROM, M. D., EGER, J. L. and HEBERT, P. D. N. (2007), DNA barcoding of Neotropical bats: species identification and discovery within Guyana. Molecular Ecology Notes, 7: 184–190. doi: 10.1111/j.1471-8286.2006.01657.x
Publication History
- Issue published online: 12 JAN 2007
- Article first published online: 12 JAN 2007
- Received 10 July 2006; revision accepted 6 November 2006
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Keywords:
- cytochrome c oxidase subunit 1;
- species diversity;
- tropics
Abstract
Sequence diversity in the cytochrome c oxidase subunit 1 gene has been shown to be an effective tool for species identification and discovery in various groups of animals, but has not been extensively tested in mammals. We address this gap by examining the performance of DNA barcodes in the discrimination of 87 species of bats from Guyana. Eighty-one of these species showed both low intraspecific variation (mean = 0.60%), and clear sequence divergence from their congeners (mean = 7.80%), while the other six showed deeply divergent intraspecific lineages suggesting that they represent species complexes. Although further work is needed to examine patterns of sequence diversity at a broader geographical scale, the present study validates the effectiveness of barcoding for the identification of regional bat assemblages, even highly diverse tropical faunas.

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