SEARCH

SEARCH BY CITATION

Keywords:

  • B. asahinai;
  • B. germanica;
  • dinucleotide microsatellite;
  • multiplex PCR;
  • tetranucleotide microsatellite;
  • trinucleotide microsatellite

Abstract

Primer sequences and initial characterization are presented for 10 microsatellite loci isolated from the German cockroach, Blattella germanica. In a sample of 30 individuals from a single population sample, all loci were polymorphic with two to 12 alleles segregating per locus and levels of observed heterozygosity ranging from 0.27 to 0.92. One locus showed a deficit of heterozygotes. Experimental conditions are described for polymerase chain reaction multiplexing, which enables the genotyping of eight loci in three electrophoretic runs consisting of one set of three and two sets of two markers. Seven primer sets cross-amplify in the related Blattella asahinai.