Characterization of four tetranucleotide and six dinucleotide microsatellite markers for use in the tropical freshwater fish Telmatherina antoniae and related species

Authors

  • RYAN P. WALTER,

    1. Great Lakes Institute for Environmental Research, University of Windsor, 401 Sunset Avenue, Windsor, Ontario, Canada N9B1G4,
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  • JENNIFER R. OVENDEN,

    1. Molecular Fisheries Laboratory, Department of Primary Industries and Fisheries, Queensland Bioscience Precinct, University of Queensland, St Lucia, 4072, Queensland, Australia
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  • DANIEL D. HEATH

    1. Great Lakes Institute for Environmental Research, University of Windsor, 401 Sunset Avenue, Windsor, Ontario, Canada N9B1G4,
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Ryan Walter, Fax: 519-971-3616; E-mail:walterj@uwindsor.ca

Abstract

Ten primer pairs were designed from two genomic libraries enriched for (GACA)4 and (GACA)7 in the sailfin silverside Telmatherina antoniae. Characterization with 57 T. antoniae individuals revealed between three and 30 alleles, with observed and expected heterozygosity values ranging from 0.47 to 0.98 and from 0.46 to 0.93, respectively. Eight of the 10 loci conformed to Hardy–Weinberg equilibrium, with no significant linkage detected between loci pairs. These microsatellite markers are intended for use in population genetic studies of T. antoniae and related fishes of the family Telmatherinidae.

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