Get access

Isolation of 22 new Haliaeetus microsatellite loci and their characterization in the critically endangered Madagascar fish-eagle (Haliaeetus vociferoides) and three other Haliaeetus eagle species

Authors

  • RUTH E. TINGAY,

    1. Natural Research Ltd, Burn O’Bennie Road, Banchory, AB31 5ZU, Scotland, UK,
    2. School of Geography, University of Nottingham, University Park, Nottingham, NG7 2RD, UK,
    3. Sheffield Molecular Genetics Facility, Department of Animal and Plant Sciences, University of Sheffield, Sheffield, S10 2TN, UK,
    Search for more papers by this author
  • DEBORAH A. DAWSON,

    1. Sheffield Molecular Genetics Facility, Department of Animal and Plant Sciences, University of Sheffield, Sheffield, S10 2TN, UK,
    Search for more papers by this author
  • JAGROOP PANDHAL,

    1. Sheffield Molecular Genetics Facility, Department of Animal and Plant Sciences, University of Sheffield, Sheffield, S10 2TN, UK,
    Search for more papers by this author
  • MICHÈLE L. CLARKE,

    1. School of Geography, University of Nottingham, University Park, Nottingham, NG7 2RD, UK,
    Search for more papers by this author
  • VICTOR A. DAVID,

    1. Laboratory of Genomic Diversity, National Cancer Institute, Frederick, Maryland, 21702-1201, USA,
    Search for more papers by this author
  • FRANK HAILER,

    1. Department of Evolutionary Biology, Evolutionary Biology Centre, Uppsala University, Norbyvägen 18d, 75236 Uppsala, Sweden,
    Search for more papers by this author
  • MELANIE CULVER

    1. Arizona Cooperative Fish & Wildlife Research Unit, USGS, University of Arizona, Tucson, AZ 85721-8801, USA
    Search for more papers by this author

  • The content of this publication does not necessarily reflect the views or policies of the Department of Health and Human Services, nor does mention of trade names, commercial products or organizations imply endorsement by the US Government.

R. Tingay, Fax: +44 (0)1330 820670; E-mail: dimlylit100@hotmail.com

Abstract

We isolated a total of 22 microsatellite loci from two Haliaeetus species: the Madagascar fish-eagle (Haliaeetus vociferoides) and the bald eagle (Haliaeetus leucocephalus). Five loci were monomorphic in both the Madagascar fish-eagle (n = 24–43) and the bald eagle (n = 2–8) but were found to be polymorphic in other Haliaeetus species. Haliaeetus loci have proved useful for investigating gene flow in Haliaeetus and Aquila eagles. Ten loci were polymorphic in the critically endangered Madagascar fish-eagle and will be used to investigate the genetic population structure and mating system in this species.

Ancillary