wombsoft is an r package that analyses individually georeferenced multilocus genotypes for the inferences of genetic boundaries between populations. It is based on the Wombling method that estimates the systemic function by looking for the local variation of the allele frequencies. This study presents an original way of estimating the systemic function, based on the local polynomial regression, and a binomial test to assess the significance of boundaries. The method applies to codominant or dominant markers and allows for missing data. The software r can be downloaded from http://www.r-project.org/ and wombsoft from http://www-leca.ujf-grenoble.fr/logiciels.htm or http://www.r-project.org/.