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Isolation and characterization of microsatellite markers for red elm (Ulmus rubra Muhl.) and cross-species amplification with Siberian elm (Ulmus pumila L.)

Authors

  • J. E. ZALAPA,

    1. Agricultural Research Service, Vegetable Crops Unit, Department of Horticulture, US Department of Agriculture, University of Wisconsin, Madison, 1575 Linden Drive, WI 53706 USA,
    2. Department of Forest Ecology and Management, University of Wisconsin, Madison, 1630 Linden Drive, WI 53706, USA
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  • J. BRUNET,

    1. Agricultural Research Service, Vegetable Crops Unit, Department of Horticulture, US Department of Agriculture, University of Wisconsin, Madison, 1575 Linden Drive, WI 53706 USA,
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  • R. P. GURIES

    1. Department of Forest Ecology and Management, University of Wisconsin, Madison, 1630 Linden Drive, WI 53706, USA
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J. E. Zalapa, Fax: (608)262-4743; E-mail: jezalapa@wisc.edu

Abstract

Ulmus pumila is an elm species, non-native to the USA that hybridizes with Ulmus rubra. In order to study the genetic structure and hybridization patterns between these two elm species, we developed 15 primer pairs for microsatellite loci in U. rubra and tested their cross-amplification in U. pumila. All 15 primers amplified in both species, 11 of which possessed species-specific alleles. Eight loci were polymorphic in U. pumila and eight in U. rubra, each with two to eight alleles per locus. In addition, five primer pairs previously developed in U. laevis and U. carpinifolia (syn. U. minor) cross-amplified and showed polymorphic loci in U. pumila and/or U. rubra. These markers will facilitate the study of genetic structure and gene flow between U. rubra and exotic, invasive U. pumila.

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