Thirty-eight single nucleotide polymorphism markers for high-throughput genotyping of chum salmon

Authors

  • CARITA M. ELFSTROM,

    1. Gene Conservation Laboratory, Division of Commercial Fisheries, Alaska Department of Fish and Game, 333 Raspberry Road, Anchorage, AK 99518, USA
    Search for more papers by this author
  • CHRISTIAN T. SMITH,

    Search for more papers by this author
    • *

      Current address: US Fish & Wildlife Service, Abernathy Fish Technology Center, 1440 Abernathy Creek Road, Longview, WA, 98632, USA.

  • LISA W. SEEB

    1. Gene Conservation Laboratory, Division of Commercial Fisheries, Alaska Department of Fish and Game, 333 Raspberry Road, Anchorage, AK 99518, USA
    Search for more papers by this author

Carita M Elfstrom, Fax: +907 267 2442; E-mail: carita_elfstrom@fishgame.state.ak.us

Abstract

We characterize 38 single nucleotide polymorphism genotyping assays for chum salmon (Oncorhynchus keta), an important species for both commercial and subsistence fisheries in western Alaska. These assays are based on the 5′-nuclease reaction and thus facilitate high-throughput genotyping with minimal optimization time. Minor allele frequency differences (Δq) among collections were between 0.01 and 0.50 resulting in per locus FST estimates of 0.00–0.08 with an average of 0.03.

Ancillary