Current address: US Fish & Wildlife Service, Abernathy Fish Technology Center, 1440 Abernathy Creek Road, Longview, WA, 98632, USA.
Thirty-eight single nucleotide polymorphism markers for high-throughput genotyping of chum salmon
Article first published online: 6 JUN 2007
Molecular Ecology Notes
Volume 7, Issue 6, pages 1211–1215, November 2007
How to Cite
ELFSTROM, C. M., SMITH, C. T. and SEEB, L. W. (2007), Thirty-eight single nucleotide polymorphism markers for high-throughput genotyping of chum salmon. Molecular Ecology Notes, 7: 1211–1215. doi: 10.1111/j.1471-8286.2007.01835.x
- Issue published online: 6 JUN 2007
- Article first published online: 6 JUN 2007
- Received 25 February 2007; revision accepted 13 April 2007
- 5′-nuclease assay;
- chum salmon;
- Oncorhynchus keta;
We characterize 38 single nucleotide polymorphism genotyping assays for chum salmon (Oncorhynchus keta), an important species for both commercial and subsistence fisheries in western Alaska. These assays are based on the 5′-nuclease reaction and thus facilitate high-throughput genotyping with minimal optimization time. Minor allele frequency differences (Δq) among collections were between 0.01 and 0.50 resulting in per locus FST estimates of 0.00–0.08 with an average of 0.03.