Primers and polymerase chain reaction conditions for DNA barcoding teleost fish based on the mitochondrial cytochrome b and nuclear rhodopsin genes

Authors

  • RAFAEL G. SEVILLA,

    1. Department of Biochemistry and Molecular Biology IV, Universidad Complutense de Madrid (UCM), Facultad de Veterinaria, 28040 Madrid, Spain,
    Search for more papers by this author
  • AMALIA DIEZ,

    1. Department of Biochemistry and Molecular Biology IV, Universidad Complutense de Madrid (UCM), Facultad de Veterinaria, 28040 Madrid, Spain,
    Search for more papers by this author
  • MICHAEL NORÉN,

    1. Swedish Museum of Natural History, Frescativaegen 40, PO Box 50007, SE-104 05, Stockholm, Sweden,
    Search for more papers by this author
  • OLIVIER MOUCHEL,

    1. French Research Institute for the Exploitation of the Sea (IFREMER), Centre de Nantes Rue de l’île d’Yeu, 44311, Nantes cedex 3, France,
    Search for more papers by this author
  • MARC JÉRÔME,

    1. French Research Institute for the Exploitation of the Sea (IFREMER), Centre de Nantes Rue de l’île d’Yeu, 44311, Nantes cedex 3, France,
    Search for more papers by this author
  • VÉRONIQUE VERREZ-BAGNIS,

    1. French Research Institute for the Exploitation of the Sea (IFREMER), Centre de Nantes Rue de l’île d’Yeu, 44311, Nantes cedex 3, France,
    Search for more papers by this author
  • HILDE VAN PELT,

    1. Netherlands Institute for Fisheries Research, Haringkade 1, 1970 AB IJmuiden, The Netherlands,
    Search for more papers by this author
  • LAURENCE FAVRE-KREY,

    1. National Agricultural Research Foundation-Fisheries Research Institute, Nea Peramos, Kavala, GR-64007, Greece
    Search for more papers by this author
  • GRIGORIOS KREY,

    1. National Agricultural Research Foundation-Fisheries Research Institute, Nea Peramos, Kavala, GR-64007, Greece
    Search for more papers by this author
  • THE FISHTRACE CONSORTIUM,

    Search for more papers by this author
    • ††

      The FishTrace Consortium (http://www.fishtrace.org) comprises 53 members from the following institutions: University Complutense of Madrid; Joint Research Centre of the European Commission; Swedish Museum of Natural History; Canarian Institute of Marine Sciences; French Research Institute for the Exploitation of the Sea; Netherlands Institute for Fisheries Research; Natural History Museum of Funchal; Natural History Museum of Tenerife; Fisheries Research Institute of Kavala; and National Natural History Museum of Paris

  • JOSÉ M. BAUTISTA

    1. Department of Biochemistry and Molecular Biology IV, Universidad Complutense de Madrid (UCM), Facultad de Veterinaria, 28040 Madrid, Spain,
    Search for more papers by this author

José M. Baustista, Fax: +34913943824; E-mail: jmbau@vet.ucm.es

Abstract

This report describes a set of 21 polymerase chain reaction primers and amplification conditions developed to barcode practically any teleost fish species according to their mitochondrial cytochrome b and nuclear rhodopsin gene sequences. The method was successfully tested in more than 200 marine fish species comprising the main Actinopterygii family groups. When used in phylogenetic analyses, its combination of two genes with different evolutionary rates serves to identify fish at the species level. We provide a flow diagram indicating our validated polymerase chain reaction amplification conditions for barcoding and species identification applications as well as population structure or haplotyping analyses, adaptable to high-throughput analyses.

Ancillary