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Characterization of microsatellite loci in the western tarnished plant bug, Lygus hesperus Knight (Hemiptera: Miridae)

Authors


O. P. Perera, E-mail: operera@msa-stoneville.ars.usda.gov

Abstract

A microsatellite-enriched partial genomic DNA library of Lygus hesperus was generated and screened by sequencing. Ten polymorphic microsatellite marker loci were characterized by genotyping 92 insect samples. The observed number of alleles ranged from three to seven with an average of 4.5 (SE ± 0.45), while the effective number of alleles ranged from 1.21 to 3.02 with an average of 2.14 (SE ± 0.20). Significant deviations from Hardy–Weinberg expectations were detected at three loci. Significant linkage disequilibrium was also detected between the loci LhMS2-54 and LhMS3-32. Seven of the L. hesperus markers could be transferred to Lygus lineolaris.

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