Characterization of microsatellite markers in mandarin orange (Citrus reticulata Blanco)

  1. Y. FROELICHER1,
  2. D. DAMBIER2,
  3. J. B. BASSENE1,
  4. G. COSTANTINO3,
  5. S. LOTFY2,4,
  6. C. DIDOUT5,
  7. V. BEAUMONT5,
  8. P. BROTTIER6,
  9. A. M. RISTERUCCI7,
  10. F. LURO3,
  11. P. OLLITRAULT2

Article first published online: 28 JUN 2008

DOI: 10.1111/j.1471-8286.2007.01893.x

Issue

Molecular Ecology Resources

Molecular Ecology Resources

Volume 8, Issue 1, pages 119–122, January 2008

Additional Information

How to Cite

FROELICHER, Y., DAMBIER, D., BASSENE, J. B., COSTANTINO, G., LOTFY, S., DIDOUT, C., BEAUMONT, V., BROTTIER, P., RISTERUCCI, A. M., LURO, F. and OLLITRAULT, P. (2008), Characterization of microsatellite markers in mandarin orange (Citrus reticulata Blanco). Molecular Ecology Resources, 8: 119–122. doi: 10.1111/j.1471-8286.2007.01893.x

Author Information

  1. 1

    Cirad, UPR Multiplication végétative, San Giuliano, F-20230 San Nicolao, France,

  2. 2

    Cirad, UPR Multiplication végétative, Avenue Agropolis, F-34398 Montpellier Cedex 5, France,

  3. 3

    Inra, Unité de Génétique et écophysiologie de la qualité des agrumes, San Giuliano, F-20230 San Nicolao, France,

  4. 4

    Inra, Station d’El Menzeh, BP 293 Kénitra, Morocco,

  5. 5

    Centre de Recherche Pernod-Ricard, 120 Avenue du Maréchal Foch, F-94015 Créteil Cedex, France,

  6. 6

    Genoscope, 2 rue Gaston Cremieux, F-91057 Evry, France,

  7. 7

    Cirad, UMR 1096, Avenue Agropolis, BP 5035, F-34032 Montpellier Cedex 5, France

* Yann Froelicher, Cirad, Fax: +33 (0)495595937; E-mail: froelicher@cirad.fr

Publication History

  1. Issue published online: 28 JUN 2008
  2. Article first published online: 28 JUN 2008
  3. Received 12 April 2007; revision accepted 4 June 2007

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Keywords:

  • Citrus;
  • genetic diversity;
  • microsatellite;
  • molecular marker;
  • Rutaceae;
  • STMS

Abstract

A dinucleotide-enriched genomic library was obtained from mandarin orange (Citrus reticulata Blanco). A subset of 101 positive clones was sequenced and primers were designed. The loci were screened for levels of variation using 26–29 wild mandarin oranges collected in Vietnam. Forty-three loci were polymorphic with the number of alleles ranging from two to 18. The observed heterozygosity (HO) and expected heterozygosity (HE) were from 0.03 to 0.96 and from 0.03 to 0.92, respectively.

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