PERMANENT GENETIC RESOURCES: Isolation of 60 polymorphic microsatellite loci in EST libraries of four sibling species of the phytopathogenic fungal complex Microbotryum

  1. T. GIRAUD1,*,
  2. R. YOCKTENG1,
  3. S. MARTHEY2,
  4. H. CHIAPELLO2,
  5. O. JONOT1,
  6. M. LOPEZ-VILLAVICENCIO1,
  7. D. M. DE VIENNE1,
  8. M. E. HOOD3,
  9. G. REFREGIER1,
  10. A. GENDRAULT-JACQUEMARD2,
  11. P. WINCKER4,
  12. C. DOSSAT4

Article first published online: 28 JUN 2008

DOI: 10.1111/j.1471-8286.2007.01967.x

Issue

Molecular Ecology Resources

Molecular Ecology Resources

Volume 8, Issue 2, pages 387–392, March 2008

Additional Information

How to Cite

GIRAUD, T., YOCKTENG, R., MARTHEY, S., CHIAPELLO, H., JONOT, O., LOPEZ-VILLAVICENCIO, M., DE VIENNE, D. M., HOOD, M. E., REFREGIER, G., GENDRAULT-JACQUEMARD, A., WINCKER, P. and DOSSAT, C. (2008), PERMANENT GENETIC RESOURCES: Isolation of 60 polymorphic microsatellite loci in EST libraries of four sibling species of the phytopathogenic fungal complex Microbotryum. Molecular Ecology Resources, 8: 387–392. doi: 10.1111/j.1471-8286.2007.01967.x

Author Information

  1. 1

    ESE, Bâtiment 360, Université Paris-Sud, 91405 Orsay, France,

  2. 2

    MIG—INRA Bâtiment 233, Domaine de Vilvert, 78350 Jouy en Josas Cedex, France,

  3. 3

    Department of Biology, Amherst College, Amherst, MA 01002, USA,

  4. 4

    Genoscope—CNRG CP 5706, 91057 Evry Cedex, France

* Correspondence: T. Giraud, Fax: +33 1 69 15 46 97; E-mail: tatiana.giraud@u-psud.fr

Publication History

  1. Issue published online: 28 JUN 2008
  2. Article first published online: 28 JUN 2008
  3. Received 17 June 2007; revision accepted 1 August 2007

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Keywords:

  • basidiomycete;
  • phytopathogen;
  • repeats;
  • transposable elements;
  • Ustilago violacea

Abstract

We report the development of 60 microsatellite markers on four species of the fungal complex Microbotryum, causing anther smut of the Caryophyllaceae. Microsatellites were found in four expressed sequence tag (EST) libraries, built from isolates of M. lychnis-dioicae, M. violaceum sensus stricto, M. lagerheimii and M. dianthorum, collected, respectively, from the plants Silene latifolia, S. nutans, S. vulgaris and Dianthus carthusianorum. Intrapopulation polymorphism was investigated using 24 isolates, and cross-amplification was explored using 23 isolates belonging to at least 10 different Microbotryum species. This study provides numerous microsatellite markers for population genetics and mapping studies.

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