PERMANENT GENETIC RESOURCES: Isolation and characterization of 12 microsatellite markers in the middle-spotted woodpecker (Dendrocopos medius)

Authors

  • M. VILA,

    1. Department of Molecular and Cell Biology, Faculty of Sciences, University of A Coruna, Campus Zapateira, E-15008, A Coruna, Spain,
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  • H. ROBLES,

    1. Laboratory of Animal Ecology, University of Antwerp, Campus Drie Eiken, Universiteitsplein 1, 26210 Wilrijk, Belgium,
    2. Department of Biodiversity and Environment Management, University of León, Campus Vegazana, E-24071, León, Spain,
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  • C. CIUDAD,

    1. Department of Biodiversity and Environment Management, University of León, Campus Vegazana, E-24071, León, Spain,
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  • P. P. OLEA,

    1. Department of Molecular Biology and Environmental Sciences, SEK University, Campus Santa Cruz la Real, E-40003 Segovia, Spain,
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  • V. BAGLIONE

    1. Department of Agro-forestry, University of Valladolid, Avenida Madrid 44, E-34004, Palencia, Spain
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Marta Vila, Fax: +34981167065; E-mail: mvila@udc.es

Abstract

Twelve polymorphic microsatellite loci were developed for Dendrocopos medius. Polymorphism was assessed for 27 individuals from the southwesternmost population of this woodpecker species. The number of alleles per locus ranged from three to seven, with observed heterozygosity values from 0.444 to 0.852. Genotypic frequencies conformed to Hardy–Weinberg equilibrium, and no evidence for linkage disequilibrium was observed. Multilocus genotypes resulting from this set of markers will be useful to determine genetic diversity and differentiation within and among habitat patches inhabited by D. medius. Three of the loci were polymorphic for Picoides articus.

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