Identification and characterization of simple sequence repeat markers from a glandular Origanum vulgare expressed sequence tag

  1. JOHANNES NOVAK1,
  2. BRIGITTE LUKAS1,
  3. KERSTIN BOLZER1,
  4. SABINE GRAUSGRUBER-GRÖGER1,
  5. JÖRG DEGENHARDT2

Article first published online: 28 JUN 2008

DOI: 10.1111/j.1471-8286.2007.02059.x

Issue

Molecular Ecology Resources

Molecular Ecology Resources

Volume 8, Issue 3, pages 599–601, May 2008

Additional Information

How to Cite

NOVAK, J., LUKAS, B., BOLZER, K., GRAUSGRUBER-GRÖGER, S. and DEGENHARDT, J. (2008), Identification and characterization of simple sequence repeat markers from a glandular Origanum vulgare expressed sequence tag. Molecular Ecology Resources, 8: 599–601. doi: 10.1111/j.1471-8286.2007.02059.x

Author Information

  1. 1

    Institute for Applied Botany, University of Veterinary Medicine, Veterinärplatz 1, A-1210 Vienna, Austria,

  2. 2

    Department of Biochemistry, Max Planck Institute for Chemical Ecology, Hans-Knöll-Straße 8, D-07745 Jena, Germany

* Johannes Novak, Fax: +43 1 250773190; E-mail: johannes.novak@vu-wien.ac.at

Publication History

  1. Issue published online: 28 JUN 2008
  2. Article first published online: 28 JUN 2008
  3. Received 26 May 2007; revision accepted 1 November 2007

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Keywords:

  • EST;
  • marjoram;
  • oregano;
  • Origanum majorana L.;
  • Origanum vulgare L.;
  • SSR

Abstract

Oregano (Origanum vulgare) and marjoram (Origanum majorana) are two sensorial distinct spices within the genus Origanum (Lamiaceae). Simple sequence repeat (SSR) markers were developed from expressed sequence tags (ESTs) of essential oil glands of O. vulgare. Thirteen EST-SSR loci were evaluated using 20 individual plants of O. vulgare and 19 plants of Origanum majorana. The number of alleles per locus ranged from one to four. All loci developed from O. vulgare successfully cross-amplified in O. majorana.

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