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Fig. S1 Bayesian consensus phylogenetic tree of Nif/Vnf/AnfDK and BchNBYZ proteins from taxa representing the primary lineages for each locus.

Fig. S2 Bayesian consensus phylogenetic tree of Vnf/Anf/NifDKEN and BchNBYZ representative loci.

Fig. S3 Rate-smoothed radial chronogram of representative Vnf/Anf/NifDKEN and BchNBYZ loci as estimated using the penalized likelihood method.

Table S1 Accession numbers for nitrogenase protein sequences used in this study.

Table S2 Accession numbers for BchNBYZ loci used in this study.

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