Data S1. Modeling the revised network for dissimilatory sulfate reduction.

Figure S1. Network of reactions in the isotope model. Similar to Fig. 1 in the main text, but including the model parameter associated with each reaction.

Figure S2. (A) Model results for the Rees-like pathway showing the isotopic compositions of intracellular sulfide, sulfite, and sulfate with Rees fractionation factor applied to α3. (B) same result with BB05 fractionation factor applied to α3.

Figure S3. Isotopic composition of intracellular sulfide (thin black dotted field), sulfite (thin grey dotted field), and the sulfanyl (thick black field), and sulfonyl (thick grey field) of trithionate in a reaction network with a thionate loop and no leaks. Thiosulfate reductase is assumed to have the fractionation factors given by Smock et  al. (1998).

Figure S4. Isotopic composition of sulfide produced by a cell with a thionate loop and various proportions of thionate leaks.

Table S1. Parameters in the branching network model.

Table S2. Assigned values for parameters in the examples given in the text.

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