Fig. S1 Venn diagrams of number of shared OTUs between each pair of samples from this study.

Fig. S2 Expansion of the Marinobacter branch of the Gammaproteobacteria tree (Fig. 4).

Fig. S3 Rarefaction curve for clone libraries from this study, calculated with MOTHUR.

Fig. S4 16S rRNA phylogenetic tree of Gammaproteobacteria clones recovered during this study, with representative Alphaproteobacteria, Zetaproteobacteria and Deferribacteres included to root the tree.

Table S1 Distribution of OTUs in clone libraries from this study. OTUs with an asterisk (*) next to their name are from the group ‘other (non-plume)’ in Fig. 2.

Table S2 Diversity indices as calculated by MOTHUR. Ranges given are 95% confidence intervals for each index.

Table S3 Relative hydrothermal component of the particulates collected [calculated as the ratio of Fe to (Fe+Mn+Al)].

Table S4 Ecophysiology of Zetaproteobacteria, Alphaproteobacteria and Gammaproteobacteria clones recovered from time points 13 and 21.


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