Cloning of Ruminococcus albus endo-β-1,4-glucanase and xylanase genes

Authors

  • M.P.M. Romaniec,

    1. Department of Biochemistry, AFRC Institute of Animal Physiology and Genetics Research, Babraham, Cambridge CB2 4AT, UK
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    • †Department of Applied Biological Sciences, Massachusetts Institute of Technology, Cambridge, MA 02139, USA

  • K. Davidson,

    1. Department of Biochemistry, AFRC Institute of Animal Physiology and Genetics Research, Babraham, Cambridge CB2 4AT, UK
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  • B.A. White,

    1. Department of Biochemistry, AFRC Institute of Animal Physiology and Genetics Research, Babraham, Cambridge CB2 4AT, UK
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    • ‡Department of Animal Sciences, University of Illinois at Urban-Champaign, Urbana, IL 61801, USA.

  • G.P. Hazlewood

    1. Department of Biochemistry, AFRC Institute of Animal Physiology and Genetics Research, Babraham, Cambridge CB2 4AT, UK
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Abstract

Two different clones expressing endogluconase activity were isolated from a plasmid gene bank constructed from Ruminococcus albus SY3 genomic DNA. Hydrolysis of xylan and lichenan by cell-free extract prepared from one of the clones may be attributable to a multifunctional protein. DNA hybridization demonstrated the absence of homology between the two endogluconase genes and a number of cellulase-related genes cloned previously from R. albus 8.

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