The use of latex agglutination tests for determining Campylobacter species

Authors

  • Wilma C. Hazeleger,

    1. Wageningen Agricultural University, Department of Food Sciences, Section of Food Chemistry and Food Microbiology, Bomenweg 2, NL 6703 HD Wageningen, The Netherlands
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  • R.R. Beumer,

    1. Wageningen Agricultural University, Department of Food Sciences, Section of Food Chemistry and Food Microbiology, Bomenweg 2, NL 6703 HD Wageningen, The Netherlands
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  • F.M. Rombouts

    1. Wageningen Agricultural University, Department of Food Sciences, Section of Food Chemistry and Food Microbiology, Bomenweg 2, NL 6703 HD Wageningen, The Netherlands
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Abstract

A comparison was made between three commercially available latex agglutination tests for the detection of Campylobacter. All tests showed clear agglutination with pure cultures of several Campylobacter strains in both the spiral and coccoid form. The Microscreen test was able to detect 10 times less cells than the Campyslide and Meritec tests. The latex tests were also applied to enrichment broth cultures of chicken products. Sixty-nine per cent of the Campylobacter positive enrichment broth cultures were positive with the Microscreen test. The Meritec test detected 63% of the positive samples. The Campyslide test detected only 15% of the positive samples and often showed non-specific agglutination.

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