Rapid extraction of high purity chromosomal DNA from Serratia marcescens

Authors

  • R. Alonso,

    1. *Servicio de Microbiologia, Hospital Ramon y Cajal, Ctra. Colmenar Km. 9,100, 28034 Madrid, Spain
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    • 1

      Departamento de Ciencias Clinicas, Universidad de Las Palmas de Gran Canaria, 38080 Las Palmas de Gran Canaria, Spain.

  • P. S. Nicholson,

    1. †Division of Hospital Infection, Central Public Health Laboratory, 61 Colindale Avenue, London NW9 5HT, UK
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  • T. L. Pitt

    1. †Division of Hospital Infection, Central Public Health Laboratory, 61 Colindale Avenue, London NW9 5HT, UK
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Abstract

Rapid non-specific degradation of Serratia marcescens DNA extracted with guanidium thiocyanate, occurred within 10 min of incubation with restriction endo-nuclease enzymes. The described modified method based on chemical and enzymatic deproteinization produced preparations of Ser. marcescens DNA of high yield and quality which did not autodegrade when incubated with restriction endonucleases.

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