Characterization of cold-active pectate lyases from psychrophilic Mrakia frigida
Article first published online: 11 APR 2005
Letters in Applied Microbiology
Volume 40, Issue 6, pages 453–459, June 2005
How to Cite
Margesin, R., Fauster, V. and Fonteyne, P.-A. (2005), Characterization of cold-active pectate lyases from psychrophilic Mrakia frigida. Letters in Applied Microbiology, 40: 453–459. doi: 10.1111/j.1472-765X.2005.01704.x
- Issue published online: 11 APR 2005
- Article first published online: 11 APR 2005
- 2004/1082: received 16 September 2004, revised 7 February 2005 and accepted 8 February 2005
- cold-active enzyme;
- Mrakia frigida;
- pectate lyase;
- psychrophilic yeast
Aims: This study was conducted to determine optimal conditions for pectate lyase (PL) production by two psychrophilic yeast strains and to compare the properties of the cold-active enzymes using mesophilic PL as reference enzyme.
Methods and Results: Two psychrophilic yeasts isolated from remote geographical locations (European Alps, north Siberia) produced extracellular cold-active PL. Both strains were identified as Mrakia frigida by analysis of ITS and large subunit (LSU) rRNA sequences. Maximum enzyme production occurred at a cultivation temperature of 1 or 5°C. The apparent optimum for enzyme activity was observed at 30°C and pH 8·5–9. The enzymes were thermolabile, but were resistant to repeated freezing and thawing.
Conclusion: We describe for the first time alkaline PL-producing representatives of the yeast species M. frigida. The two strains produce cold-active PL with similar properties, but have a different enzyme production pattern.
Significance and Impact of the Study: The enzymes described in this study could be useful for a wide range of applications, such as low-temperature pretreatment of wastewater containing pectic substances.