Rapid detection of viruses using electrical biochips and anti-virion sera
Article first published online: 11 APR 2005
Letters in Applied Microbiology
Volume 40, Issue 6, pages 479–485, June 2005
How to Cite
Łoś, M., Łoś, J.M., Blohm, L., Spillner, E., Grunwald, T., Albers, J., Hintsche, R. and Węgrzyn, G. (2005), Rapid detection of viruses using electrical biochips and anti-virion sera. Letters in Applied Microbiology, 40: 479–485. doi: 10.1111/j.1472-765X.2005.01706.x
- Issue published online: 11 APR 2005
- Article first published online: 11 APR 2005
- 2004/1273: received 5 November 2004, revised 19 February 2005 and accepted 21 February 2005
- bacteriophage contamination of bacterial cultures;
- bacteriophage-encoded bacterial toxins;
- phage lambda;
- phage M13;
- quantitative detection of viruses
Aims: Rapid detection and quantification of viruses is crucial in clinical practice, veterinary medicine, agriculture, basic research as well as in biotechnological factories. However, although various techniques were described and are currently used, development of more rapid, more sensitive and quantitative methods seems to be still important.
Methods and Results: Here we describe a method for rapid detection of viruses (using bacteriophages as model viruses), based on electrical biochip array technology with the use of antibodies against capsid proteins.
Conclusions: Using the procedure developed in this work, we were able to detect 2 × 104 virions on the chip. The whole assay procedure takes c. 50 min and the assay is quantitative.
Significance and Impact of the Study: This procedure may be useful in various approaches, including detection of bacteriophage contamination in bioreactors and possibly detection of toxin gene-bearing phages or other viruses in food samples.